Peptide glossary · Analysis
Area percent
The unit of HPLC purity: the main peak's area divided by the area of all peaks. Anything the detector does not see, such as water or salts, is not counted.
In the lab
The figure depends on the wavelength. A measurement at 280 nm alone misses impurities without aromatic residues and can make purity look too high.
Read morePeptide content vs purity: the difference explainedMore terms: Analysis
- AggregationPeptide molecules clumping together, often with hydrophobic sequences.
- Amino acid analysisA method in which the peptide is fully hydrolysed and the individual amino acids are measured.
- ChromatogramThe curve an HPLC run produces: detector signal over time.
- CounterionThe ion that balances the charge of a peptide salt, such as trifluoroacetate or acetate.
- DeamidationA degradation route in which asparagine or glutamine lose their amide group.
- EndotoxinsCell wall components of certain bacteria that can strongly distort cell experiments.
For laboratory research use only. Not for use in humans or animals.