Peptide glossary · Analysis
Retention time
The time after which a substance leaves the HPLC column. It depends on column, gradient and hydrophobicity, and is only comparable within the same method.
In the lab
A stable retention time over several runs shows the method is reproducible. On its own it says little about a substance's identity.
More terms: Analysis
- AggregationPeptide molecules clumping together, often with hydrophobic sequences.
- Amino acid analysisA method in which the peptide is fully hydrolysed and the individual amino acids are measured.
- Area percentThe unit of HPLC purity: the main peak's area divided by the area of all peaks.
- ChromatogramThe curve an HPLC run produces: detector signal over time.
- CounterionThe ion that balances the charge of a peptide salt, such as trifluoroacetate or acetate.
- DeamidationA degradation route in which asparagine or glutamine lose their amide group.
For laboratory research use only. Not for use in humans or animals.