Peptide glossary · Analysis
RP-HPLC
Reversed-phase HPLC with a non-polar column and a gradient of water and acetonitrile, usually with 0.1% trifluoroacetic acid. More hydrophobic peptides come out later. It is the standard method for peptide purity.
In the lab
For coupling with mass spectrometry, TFA is often replaced by formic acid, because TFA suppresses the signal. Chromatograms from the two can differ.
Read moreTest methodsMore terms: Analysis
- AggregationPeptide molecules clumping together, often with hydrophobic sequences.
- Amino acid analysisA method in which the peptide is fully hydrolysed and the individual amino acids are measured.
- Area percentThe unit of HPLC purity: the main peak's area divided by the area of all peaks.
- ChromatogramThe curve an HPLC run produces: detector signal over time.
- CounterionThe ion that balances the charge of a peptide salt, such as trifluoroacetate or acetate.
- DeamidationA degradation route in which asparagine or glutamine lose their amide group.
For laboratory research use only. Not for use in humans or animals.