Peptide glossary · Analysis
Oxidation
A degradation route mainly affecting methionine, cysteine and tryptophan. Oxidised methionine shows in mass spectrometry at +16 Da. Keep air exposure low.
In the lab
DMSO can oxidise methionine and cysteine. For such peptides DMF is often the better organic solvent.
Read moreStoring peptides: fridge, freezer and lyophilisateMore terms: Analysis
- AggregationPeptide molecules clumping together, often with hydrophobic sequences.
- Amino acid analysisA method in which the peptide is fully hydrolysed and the individual amino acids are measured.
- Area percentThe unit of HPLC purity: the main peak's area divided by the area of all peaks.
- ChromatogramThe curve an HPLC run produces: detector signal over time.
- CounterionThe ion that balances the charge of a peptide salt, such as trifluoroacetate or acetate.
- DeamidationA degradation route in which asparagine or glutamine lose their amide group.
For laboratory research use only. Not for use in humans or animals.